T0467 activates parkin mitochondrial translocation in a PINK1-dependent manner in vitro. T0467 do not induce mitochondrial accumulation of PINK1in dopaminergic neurons. T0467 is a potential compound for PINK1-Parkin signaling activation, and can be used for parkinson's disease and related disorders research.

Product information

CAS Number: 859518-94-8

Molecular Weight: 441.49

Formula: C24H26F3N5

Chemical Name: (S)-N-(3, 5-difluorophenethyl)-4-(2-fluoro-5-((2-methylpiperazin-1-yl)methyl)phenyl)pyrimidin-2-amine

Smiles: C[C@H]1CNCCN1CC1=CC(C2=CC=NC(NCCC3C=C(F)C=C(F)C=3)=N2)=C(F)C=C1

InChiKey: GNYKYGZGDGZPOK-INIZCTEOSA-N

InChi: InChI=1S/C24H26F3N5/c1-16-14-28-8-9-32(16)15-18-2-3-22(27)21(12-18)23-5-7-30-24(31-23)29-6-4-17-10-19(25)13-20(26)11-17/h2-3,5,7,10-13,16,28H,4,6,8-9,14-15H2,1H3,(H,29,30,31)/t16-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

T0467 (2.5-20 μM; 3 hours) stimulates the mitochondrial translocation of GFP-Parkinover 12 μM in HeLa/GFP-Parkin cells. When HeLa/GFP-Parkin cells are treated with 20 μM T0467 for 3 h, GTP-Parkin is translocated to the mitochondria in approximately 21% of cells. T0467 does not show obvious toxicity in Drosophila at concentrations <50 μM. All cpds examined mitigated the PINK1 inactivation-mediated larval locomotion defects and mitochondrial morphological defects and reduced ATP production. T0467 and KTP improved the mitochondrial Ca2+ response in Drosophila larval muscles.

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