Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins.

Product information

CAS Number: 1146592-99-5

Molecular Weight: 1049.04

Formula: C43H68N8O22

Chemical Name: 2, 2', 2'', 2''', 2'''', 2'''''-(((11-(6-aminohexanoyl)-1, 4, 8, 11-tetraazacyclotetradecane-1, 4, 8-triyl)tris(1-carboxy-4-oxobutane-4, 1-diyl))tris(azanetriyl))hexaacetic acid

Smiles: NCCCCCC(=O)N1CCN(CCCN(CCN(CCC1)C(=O)CCC(C(O)=O)N(CC(O)=O)CC(O)=O)C(=O)CCC(C(O)=O)N(CC(O)=O)CC(O)=O)C(=O)CCC(C(O)=O)N(CC(O)=O)CC(O)=O

InChiKey: SRHQOOQNBLZULK-UHFFFAOYSA-N

InChi: InChI=1S/C43H68N8O22/c44-13-3-1-2-6-31(52)45-14-4-15-47(33(54)11-8-29(42(70)71)50(24-37(60)61)25-38(62)63)20-21-48(34(55)12-9-30(43(72)73)51(26-39(64)65)27-40(66)67)17-5-16-46(19-18-45)32(53)10-7-28(41(68)69)49(22-35(56)57)23-36(58)59/h28-30H,1-27,44H2,(H,56,57)(H,58,59)(H,60,61)(H,62,63)(H,64,65)(H,66,67)(H,68,69)(H,70,71)(H,72,73)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Tris-NTA, which in presence of Ni2+ ions can interact with a His10-tag fused to the intracellular C terminus of the receptor. Whereas the Tris-NTA group shows an affinity Kd to bind the His10-tag of ~10-30 nM.

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