Bid Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90135-50, BS90135-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55957(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC, IP,

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: Members of the Bcl-2 family of proteins interact to regulate programmed cell death, or apoptosis. Various homodimers and heterodimers formed by proteins in this family can either promote or inhibit apoptosis. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect on certain hematopoietic cell lines following growth factor withdrawal. Additional apoptotic inhibitors in this family include A1, Bag-1, Bcl-w, Bcl-x and Mcl-1. Pro-apoptotic members of this family include Bax, Bad, Bak, Bik (NBK) and BID. BID contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax. BID is localized predominantly in the cytosol and is also present in membrane fractions. It is highly expressed in kidney and can also be detected in brain, spleen, liver, testis and lung.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Bid polyclonal antibody detects endogenous levels of Bid protein.

Bioworld Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bid on human thymus cells lysates using anti-Bid antibody at 1/500 dilution., ICC staining Bid in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BID, Apoptic death agonist, Apoptotic death agonist BID, BH3 interacting domain death agonist, BH3 interacting domain death agonist p11, BH3 interacting domain death agonist p13, BH3 interacting domain death agonist p15, BH3-interacting domain death agonist p11, BID, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, BID, Desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p11 BID, p13 BID, p15 BID, p22 BID,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified