Aldolase Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90047-50, BS90047-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04075(Human) P05064(Mouse) P05065(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, ICC IF, IHC

All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200

Background: Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A; the liver-specific Aldolase B; and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldo-lase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Aldolase polyclonal antibody detects endogenous levels of Aldolase protein.

Bioworld Molecular Weight: 39 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Hela
Lane 3: Mouse liver
Lane 4: Rat liver
Lane 5: Rat spleen, ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ALDOA, ALDA, ALDA, Aldo1, ALDOA, ALDOA, Aldolase 1, Aldolase A, Aldolase A fructose bisphosphatase, Aldolase A fructose bisphosphate, Aldolase, fructose-bisphosphate A, Epididymis secretory sperm binding protein Li 87p, FRUCTOALDOLASE A, Fructose 1 6 bisphosphate triosephosphate lyase, Fructose bisphosphate aldolase A, Fructose bisphosphate aldolase, FRUCTOSE-1, 6-BISPHOSPHATE ALDOLASE A, Fructose-bisphosphate aldolase A, Fructose-bisphosphate aldolase A Muscle-type, GSD12, HEL S 87p, Lung cancer antigen NY LU 1, Lung cancer antigen NY-LU-1, MGC10942, MGC17716, MGC17767, Muscle type aldolase, Muscle-type aldolase, RNALDOG5,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified