EEF1B2 Polyclonal Antibody

Catalogue Numbers: BS5708-50, BS5708-100

Sizes: 50µl, 100µl

Alternative Name: Elongation factor 1-beta; EF-1-beta; EEF1B; EF1B; EF-1 β; EF-1β; EF1β; EF1-β

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24534

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complex is composed of four subunits: EF-1 α, EF-1 β, EF-1 δ and EF-1 γ. EF-1 β is a 225 amino acid member of the EF-1 β/EF-1 δ protein family and contains one GST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange of GDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 β result in three transcript variants that differ only in the 5' UTR.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EEF1B2 polyclonal antibody detects endogenous levels of EEF1B2 protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EEF1B2 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 142-187 of Human EEF1B2.

Conjugate: Unconjugated

Modification: Unmodified