DIABLO Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90408-50, BS90408-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NR28(Human) Q9JIQ3(Mouse) Unigene:9090(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The activation of caspases is a key regulatory step in apoptosis. Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation. Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway. Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases. In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: DIABLO polyclonal antibody detects endogenous levels of DIABLO protein.

Bioworld Molecular Weight: 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: MCF-7, ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Smac, DIABLO, 0610041G12Rik, DBLOH, DBOH, DFNA64, diablo, Diablo homolog (Drosophila), Diablo homolog, Diablo homolog mitochondrial, Diablo IAP binding mitochondrial protein, Diablo like protein, DIABLO S, Direct IAP binding protein with low pI, Direct IAP-binding protein with low pI, FLJ10537, FLJ25049, mitochondrial, Mitochondrial Smac protein, Second mitochondria derived activator of caspase, Second mitochondria-derived activator of caspase, SMAC 3, Smac, Smac protein, SMAC3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified