Recombinant Human HER2/ErbB2 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032988-10, PKSH032988-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: HER2; ErbB2

Target Synonym: Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1; CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; TKR1

UNIProt ID: P04626

Research Areas: Signal Transduction; Tags & Cell Markers; Cancer; immunology;

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P04626

Background: Human epidermal growth factor receptor 2 (HER2) is a type of membrane glycoprotein; and belongs to the epidermal growth factor (EGF) receptor family. HER2 plays a key role in development; cell proliferation and differentiation. HER2 has been reported to associate with malignancy and a poor prognosis in numerous carcinomas; including breast; prostate; ovarian; lung cancers and so on. HER2 is activated by dimerization and not activated by EGF; TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.It is heterodimer with EGFR; ERBB3 and ERBB4. HER2 associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. It implicated in transcriptional activation of CDKN1A and the function of the protein involves STAT3 and SRC. And also it involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Sequence: Thr 23-Thr652

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 70.1 kDa

Observed MW: 90-120 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only