TOM20 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9924M-50, BS9924M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15388

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF, IHC, IP,

All Applications: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:20-1:50
IP: 1:50-1:200

Background: The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TOM20 Rabbit monoclonal antibody detects endogenous levels of TOM20 protein.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution., Immunohistochemistry of paraffin-embedded human colon using TOM20 antibody at dilution of 1:100 (40x lens)., Immunohistochemistry of paraffin-embedded rat ovary using TOM20 antibody at dilution of 1:100 (40x lens)., Immunofluorescence analysis of HeLa cells using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: TOMM20; MAS20; MOM19; TOM20

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).

Conjugate: Unconjugated

Modification: Unmodified