NMDAR2B(Phospho-Tyr1336)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65150-50, BS65150-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q13224

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Background: glutamate ionotropic receptor NMDA type subunit 2B(GRIN2B) Homo sapiens N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phospho-NMDAε2 (Y1336) Polyclonal Antibody detects endogenous levels of NMDAε2 protein only when phosphorylated at Y1336.

Bioworld Molecular Weight: ~ 150 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of 293T-UV cells using Phospho-NMDAε2 (Y1336) Polyclonal Antibody diluted at 1：500, Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30', using NMDAR2B (Phospho-Tyr1336) Antibody. The Lane on the right is blocked with the phospho peptide., Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1336) Antibody. The picture on the right is blocked with the phospho peptide., Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NMDAR2B (Phospho-Tyr1336) Antibody

Alternative Name: GRIN2B; NMDAR2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; N-methyl-D-aspartate receptor subunit 3; NR3; hNR3

Immunogen: The antiserum was produced against synthesized peptide derived from human NMDAR2B around the phosphorylation site of Tyr1336. AA range:1302-1351

Conjugate: Unconjugated

Modification: Phosphorylation