Recombinant Human SEPHS1 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033016-10, PKSH033016-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: SEPHS1

Target Synonym: Selenide; water dikinase 1; Selenium donor protein 1; Selenophosphate synthase 1; SEPHS1; SELD; SPS; SPS1

UNIProt ID: P49903

Research Areas: Cell biology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P49903

Background: Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily. It has four different isoforms by alternative splicing. Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased, which Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. SEPHS1 can be activated by phosphate ions and by potassium ions. It can synthesize synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.

Sequence: Met 1-Ser392

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 25mM Tris.HCl, pH7.3, 100mM glycine, 10% glycerol.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 43.9 kDa

Observed MW: 42 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only