Free Cholesterol (FC) Colorimetric Assay Kit

Sizes: 48T, 96T, 500 Assays

Catalogue Numbers: E-BC-K004-M-48, E-BC-K004-M-96, E-BC-K004-M-500

Citations, Manuals and MSDS Available upon request.

Target Synonym: FC

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: Serum, plasma, tissue

Assay Time: 20 min

Detection Type: Colorimetric method

Sensitivity: 0.07 mmol/L

Detection Range: 0.07-24 mmol/L

Test Principle: Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.

Inter CV: 5.2

Intra CV: 1.9

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag