Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Size: 100 Assays

Catalogue Number: E-BC-K236-S-100

Citations, Manuals and MSDS Available upon request.

Target Synonym: AST;GOT

Research Areas: Liver and Renal Function

Result Type: Enzyme Activity

Detection Instrument: Spectrophotometer

Tested Sample Types: Serum, plasma, animal tissue

Assay Time: 100 min

Detection Type: Colorimetric method

Sensitivity: 0.38 IU/L

Detection Range: 0.38-72.30 IU/L

Test Principle: AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Inter CV: 4.9

Intra CV: 4.2

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag