BNP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67729-50, BS67729-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16860

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF/ICC: 1:50~1:200

Background: This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BNP polyclonal antibody detects endogenous levels of BNP protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Mouse brain, using BNP antibody at 1:1000 dilution., Immunohistochemistry of paraffin-embedded mouse heart using BNP pAb at dilution of 1:200 (40x lens)., Immunofluorescence analysis of mouse heart cells using BNP pAb at dilution of 1:150 (40x lens)., Immunofluorescence analysis of rat heart cells using BNP pAb at dilution of 1:150 (40x lens).

Alternative Name: NPPB; BNP

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human BNP (NP_002512.1).

Conjugate: Unconjugated

Modification: Unmodified