HHAT Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS76159-50, BS76159-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q5VTY9 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB All Applications: WB,1:500 - 1:2000 Background: 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).[supplied by OMIM, Jul 2002] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using HHAT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 63kDa Alternative Name: HHAT; MART2; SKI1; Skn Immunogen: A synthetic peptide of human HHAT(NP_060664.2). Conjugate: Unconjugated Modification: Unmodified