POLE3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8241-50, BS8241-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NRF9

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF

All Applications: WB: 1:500~1:2000
IF: 1:50~1:200

Background: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators . CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex),which increases the accessibility of nucleosomal DNA in an ATP-dependent manner . Unlike other known chromatin remodelling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing . This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array . In the presence of CHRAC, the nucleosomal ATPase ISWI catalyses several ATP-dependent transitions of chromatin structure .

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: POLE3 polyclonal antibody detects endogenous levels of POLE3 protein.

Bioworld Molecular Weight: ~ 20-25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of POLE3 antibody., Immunofluorescence analysis of U2OS cell using POLE3 antibody., Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser., Western blot analysis of extracts from normal (control) and POLE3 knockout (KO) 293T cells, using POLE3 antibody.

Alternative Name: DNA polymerase epsilon subunit 3; Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17; POLE3; CHRAC17

Immunogen: Recombinant full length Human POLE3.

Conjugate: Unconjugated

Modification: Unmodified