NPHS1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-12592-60, E-AB-12592-120, E-AB-12592-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: NPHS1

Target Synonym: CNF; Nephrin; Nephrosis 1 congenital Finnish type; Nephrosis 1; congenital; Finnish type (nephrin); NPHN; NPHN; NPHS 1; Nphs1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor

Research Areas: Cancer, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O60500

Background: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.

Concentration: 0.5 mg/mL

Immunogen: Synthetic peptide of human NPHS1

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:25-1:100

Calculated MW: 135kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only