MEK1 (phospho-S218/S222) Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9945M-50, BS9945M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02750

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MEK1 protein only when phosphorylated at Ser218 and/or Ser222.

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MEK1 (phospho-S218/S222) Rabbit mAb at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-MEK1(S218/S222) antibody. Counter stained with hematoxylin., ICC staining Phospho-MEK1(S218/S222) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Phosphorylation