GYPA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8042-50, BS8042-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02724

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB 1:500 - 1:2000

Background: Glycophorins A, B and C are sialoglycoproteins of the human erythrocyte membrane, which bear the antigenic determinants for the MN, Ss and Gerbich blood groups, respectively. Glycophorins span the membrane once and present their amino-terminal end to the extracellular surface of the human erythrocyte. The genetic array of expressed glycophorin surface antigens on erythrocytes defines the blood group phenotype of the individual. The human Glycophorin A gene maps to chromosome 4q31.21, contains 7 exons which are 97% homologous to Glycophorin B, and encodes a 150 amino acid protein. The human Glycophorin B gene maps to chromosome 4q31.21 and encodes a 91 amino acid protein. The human Glycophorin C gene maps to chromosome 2q14.3 and contains four exons. Glycophorin C transcript can generate two protein isoforms. Isoform 1 includes all 4 exons and encodes the full length 128 amino acid protein. Isoform 2 is missing exon 2 and encodes a 109 amino acid protein, which specifies the Yus subtype of the Gerbich phenotype.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GYPA polyclonal antibody detects endogenous levels of GYPA protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of various cell lines, using GYPA antibody at 1:1000 dilution.

Alternative Name: Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD_antigen=CD235a; GYPA; GPA

Immunogen: Recombinant full length Human GYPA.

Conjugate: Unconjugated

Modification: Unmodified