Recombinant Human NRAS Protein (His Tag)

Size: 100μg

Catalogue Numbers: PKSH030915-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: NRAS

Target Synonym: ALPS4; CMNS; N-ras; NCMS; NRAS1; NS6

UNIProt ID: P01111

Research Areas: Signal Transduction; Tags & Cell Markers; Cancer; epigenetics and nuclear signal;

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

Accession: P01111

Background: NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).

Sequence: Met 1-Cys186

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 23 kDa

Observed MW: 24 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only