CD34 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS65854-50, BS65854-100 Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Swiss-Prot: Q64314 Host: Rabbit Reactivity: Human, Mouse, Rat, Applications: WB All Applications: WB=1:500-2000 Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. Purification and Purity: affinity purified by Protein A Storage and Stability: Store at 4°C short term. Aliquot and store at -27°C long term. Avoid freeze-thaw cycles. Specificity: CD34 Polyclonal Antibody detects endogenous levels of CD34 protein. Bioworld Molecular Weight: ~120 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Primary: Anti-CD34 at 1/1000 dilution Alternative Name: CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN; Hematopoietic Stem Cell Marker; Endothelial Markers; Tumor biomarkers. Immunogen: KLH conjugated synthetic peptide derived from human CD34:201-300/382 Conjugate: Unconjugated Modification: Unmodified