MAP3K4 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-91655-60, E-AB-91655-120, E-AB-91655-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: MAP3K4

Target Synonym: MAP3K4; MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412

Research Areas: Neuroscience, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Rat

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q9Y6R4

Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.

Concentration: 1mg/mL

Immunogen: A synthetic peptide of human MAP3K4

Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:100-1:200

Calculated MW: 177kDa/181kDa

Observed MW: 170kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only