DPNB-ABT594 is a nitrobenzyl-caged ABT594 (HY-14316A) and activates nAChRs containing the α4β2 subunits with good selectivity than the α7 subunit. DPNB-ABT594 can be used to map the distribution of nAChRs on neurons of the medial habenula (MHb) and helps to gain a deeper understanding of the nAChR‐mediated Ca2+ signalling in the MHb.

Product information

Molecular Weight: 672.16

Formula: C31H46ClN3O11

Chemical Name: 5-[[(2R)-1-[1-[4, 5-bis[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]-2-nitrophenyl]ethyl]azetidin-2-yl]methoxy]-2-chloropyridine

Smiles: CC(C1=CC(OCCOCCOCCOC)=C(C=C1[N+]([O-])=O)OCCOCCOCCOC)N1CC[C@@H]1COC1=CN=C(Cl)C=C1

InChiKey: SJPLSQONRZLFRL-WUBHUQEYSA-N

InChi: InChI=1S/C31H46ClN3O11/c1-24(34-7-6-25(34)23-46-26-4-5-31(32)33-22-26)27-20-29(44-18-16-42-14-12-40-10-8-38-2)30(21-28(27)35(36)37)45-19-17-43-15-13-41-11-9-39-3/h4-5,20-22,24-25H,6-19,23H2,1-3H3/t24?,25-/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

DPNB‐ABT594 (20 μM) exhibits large inward currents and Ca2+ transients in response to laser uncaging at 410 nm at the soma, which increases linearly with energy dosage as expected for one‐photon excitation (1 mW, 0.5-3 ms). DPNB‐ABT594 is suitable for one- or two-photon photolysis, it evokes large inward currents and Ca2+ transients on cell bodies and dendrites of medial habenular neurons in mouse brain slices, following exposure to 410 nm light.

Products are for research use only. Not for human use.