Recombinant Mouse Interleukin-2/IL-2 Protein (Active)

Sizes: 20μg, 100μg

Catalogue Numbers: PKSM040308-20, PKSM040308-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: Interleukin-2; IL-2

Target Symptom: IL2; Aldesleukin; Interleukin 2; Interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture; ELISA

Fusion Tag: None

UNIProt ID: P04351

Accession: P04351

Background: Interleukin-2, also known as T-cell growth factor, TCGF, Aldesleukin and IL2, is a secreted protein which belongs to the IL-2 family. Interleukin-2 / IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 is normally produced by the body during an immune response. Antigen binding to the T cell receptor (TCR) stimulates the secretion of Interleukin-2 / IL-2, and the expression of IL-2 receptors IL-2R. The IL-2 / IL-2R interaction then stimulates the growth, differentiation and survival of antigen-selected cytotoxic T cells via the activation of the expression of specific genes. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The World Reference Standard for Interleukin-2 / IL-2 is produced by the National Institute of Biological Standards and Control in the UK. A recombinant form of Interleukin-2 / IL-2 for clinical use is manufactured by Chiron Corporation with the brand name Proleukin. It has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines. The use of Interleukin-2 / IL-2 in HIV therapy has been found to be ineffective.

Activity: 1. Measured by its ability to bind recombinant human IL2Ra-Fc in a functional ELISA.
2. Measured in a cell proliferation assay using CTLL2. The ED50 for this effect is typically 0.2-1.0 ng/mL.

Sequence: Ala 21-Gln 169

Purity: > 92 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, 2mM DTT, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 17.4 kDa

Observed MW: 19 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only