Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74222-50, BS74222-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15759/P53778/O15264/Q16539

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Mapk14 (phosphoT180/Y182) monoclonal antibody is provided, clone number M139 is a monoclonal antibody prepared from mice, its clone number is M139. Mapk14 is also known as CSBP, CSPB1, Csbp1, Csbp2, Exip, Hog, MGC105413, Mxi2, Prkm14, Prkm15, RK, Sapk2A, p38, p38Hog, p38alpha; The full name of its gene is Mitogen Activated Protein Kinase 14.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 43KDa

Immunogen: A synthetic phosphorylated peptide around T180 & Y182 of human MAPK14MAPK11(NP_002742.3).

Conjugate: Unconjugated

Modification: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of C6 cells, using Phospho-MAPK14-T180/Y182+MAPK11-T180/Y182+MAPK13-T180/Y182+MAPK12-T183/Y1845 antibody at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.