Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F032-48, E-BC-F032-96

Citations, Manuals and MSDS Available upon request.

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence Microplate Reader

Tested Sample Types: Serum, plasma, animal tissue, cells

Assay Time: 20 min

Detection Type: Fluorescence method

Sensitivity: 0.12 μmol/L

Detection Range: 0.12-30 μmol/L

Test Principle: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE) . Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.

Inter CV: 7.3

Intra CV: 1.7

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag