c-Met Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90313-50, BS90313-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08581(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity. Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines . The c-Met gene product has been identified as the cell-surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: c-Met polyclonal antibody detects endogenous levels of c-Met protein.

Bioworld Molecular Weight: 155 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Met on different lysates using anti-Met antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2, ICC staining Met in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HGF receptor, SF receptor, AUTS9, c met, D249, Hepatocyte growth factor receptor, HGF, HGF receptor, HGF/SF receptor, HGFR, MET, Met proto oncogene tyrosine kinase, MET proto oncogene, receptor tyrosine kinase, Met proto-oncogene (hepatocyte growth factor receptor), Met proto-oncogene, Met protooncogene, MET, Oncogene MET, Par4, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified