SDHA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91214-50, BS91214-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31040(Human) Q8K2B3(Mouse) Q920L2(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebrafish

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SDHA polyclonal antibody detects endogenous levels of SDHA protein.

Bioworld Molecular Weight: 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution., ICC staining SDHA in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Fp, SDHA, CMD1GG, DHSA, Flavoprotein subunit of complex II, Fp, PGL5, SDH 2, SDH1, SDH2, SDHA, SDHF, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, Succinate dehydrogenase complex flavoprotein subunit A, Succinate dehydrogenase complex flavoprotein subunit, Succinate dehydrogenase complex flavoprotein subunit precursor, Succinate dehydrogenase complex subunit A, Succinate dehydrogenase complex subunit A flavoprotein (Fp), Succinate dehydrogenase complex subunit A flavoprotein,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified