AATF (N16) Polyclonal Antibody

Catalogue Numbers: BS2152-50, BS2152-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NY61

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Che-1 (protein AATF, apoptosis-antagonizing transcription factor) is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, Che-1 may function as a general inhibitor of the histone deacetylase HDAC1. Che-1 binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of Che-1. It also antagonizes PAR4 (prostate apoptosis response 4) mediated induction of aberrant amyloid peptide production in Alzheimer disease (AD; presenile and senile dementia). PAR4 is a leucine zipper protein that is pro-apoptotic and associated with neuronal degeneration in AD. Che-1 interaction with
PAR4 suggests that it might directly or indirectly participate in regulation of PAR4 activity. Che-1 also co-localizes with PAR4 in both cytoplasmic and nuclear compartments, and it interacts directly and selectively with PAR4 via the leucine zipper domain in neural cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: AATF (N16) polyclonal antibody detects endogenous levels of AATF protein.

Extra Notes: Western blot (WB) analysis of AATF (N16) polyclonal antibody at 1:500 dilution
Lane1:H1792 whole cell lysate(20ug)
Lane2:HEK293T whole cell lysate(20ug)
Lane3:PC12 whole cell lysate(40ug)
Lane4:CT26 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 63 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein AATF; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; AATF; CHE1; DED; HSPC277

Immunogen: Synthetic peptide, corresponding to amino acids 10-60 of Human AATF.

Conjugate: Unconjugated

Modification: Unmodified