RmesFect™Stem transfection reagent principal advantages:

• Highly efficient : achieve mRNA delivery in all cells
• Ready-to-use: no need for additional buffer
• Low nucleic acid amount : minimized toxicity
• Protects mRNA against degradation
• Compatible with any culture medium
• Serum compatible : Medium changed not required

Sizes:

• 500µL (RS30500): 125-250 transfections with 1 µg of mRNA
• 1 mL (RS31000): 250-500 transfections with 1 µg of mRNA
• 5 mL (RS35000): 1250-2500 transfections with 1 µg of mRNA

Application

• Ideal for your mRNA transfection applications in stem cells:
• mRNA vaccines
• Embryonic and multipotent stem cells transfection
• Regenerative medicine
• Faster cell reprogramming : allowing daily transfections for iPS generation

RECOMMENDED FOR: Transfection of mRNA in stem cells.

Results

Figure 1: Several Stem cells were transfected using RmesFect Stem. Complexes were prepared as followed: mRNA encoding GFP protein (0.25 μg for human hMSC and 0.5 μg for MEF and hAdSC) was mixed with RmesFect Stem transfection reagent (ratio 2:1 for hMSC and 4:1 for MEF and hAdSC). After 20 min of incubation at room temperature, the complexes were added onto the cells in a dropwise manner. 24 H after, transfection efficiency was measured by  FACS analysis.

Figure 2: Several Stem cells were transfected using RmesFect Stem. 24 H after, transfection efficiency was measured by fluorescence microscopy.