LGR5 (9E10) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB8017-50, MB8017-100

Product: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Swiss-Prot: O75473

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IF, IP, IHC FC

All Applications: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul

Background: G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G protein-coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: LGR5 mAb detects endogenous levels of LGR5 protein.

Bioworld Molecular Weight: 99.5 kDa(Predicted)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: HEK293T cells were transfected with the pCMV6-ENTRY control (Left Lane) or pCMV6-ENTRY LGR5 (Right Lane) cDNA for 48 hrs and lysed., Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5., Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody.

Alternative Name: Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38

Immunogen: Recombinant LGR5 protein, corresponding to amino acids 250-550 of Human LGR5.

Conjugate: Unconjugated

Modification: Unmodified