ERK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90471-50, BS90471-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27361(Human) Q63844(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p12-p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ERK1 polyclonal antibody detects endogenous levels of ERK1 protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: K562, ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAPK3, ERK 1, ERK, ERK-1, ERK1, ERT 2, ERT2, Extracellular Signal Regulated Kinase 1, Extracellular signal related kinase 1, Extracellular signal-regulated kinase 1, HGNC6877, HS44KDAP, HUMKER1A, Insulin Stimulated MAP2 Kinase, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP Kinase, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK, MAPK1, Mapk3, MGC20180, Microtubule Associated Protein 2 Kinase, Microtubule-associated protein 2 kinase, Mitogen Activated Protein Kinase 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, MK03, OTTHUMP00000174538, OTTHUMP00000174541, p44 ERK1, p44 MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM 3, PRKM3, Protein Kinase Mitogen Activated 3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified