Chikungunya Virus E Protein ectodomain Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0149P-50, NCP0149P-100

Swiss-Prot: ABN04192.1

Host: Rabbit

Reactivity: CHIKV-E

Applications: WB, ELISA

All Applications: WB: 1:500~1:2000
ELISA: 1:5000~10000

Background: Chikungunya virus (CHIKV) belongs to the Semliki Forest antigenic group of the genus Alphaviridae, which includes other arthritogenic alphaviruses, such as o’nyong-nyong, Ross River, Barmah Forest, and Mayaro viruses.Its genome is closely related to that of o’nyong-nyong virus, and consists of a single 11.8-kbp strand of positive sense RNA, which encodes a 2472 amino acid nonstructural and a 1244 amino acid structural polyprotein.3 The polyproteins give rise to the four nonstructural proteins (nsP1-4) that make up the viral replication machine, and five structural proteins. Each spherical viral particle is approximately 70 nm in diameter and is comprised of a strand of genomic RNA, encapsidated by capsid (C) proteins, surrounded by a host cell–derived lipid bilayer spiked with heterodimers of envelope proteins E1 and E2.4 The other two structural proteins, 6K and E3, are leader peptides for E1 and E2, respectively, and are not observed in abundance in the mature virion.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Chikungunya virus E protein ectodomain polyclonal antibody detects endogenous levels of CHIKV-E protein.

Note: For research use only, not for use in diagnostic procedure.

Immunogen: Recombinant protein of CHIKV-E.

Conjugate: Unconjugated

Modification: Unmodified