Ubiquitin Conjugating Enzyme E2 A/B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16047-50, BZ16047-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P49459/P63146

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 17 kDa; Observed MW: 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ubiquitin Conjugating Enzyme E2 A/B in Jurkat lysates using UBE2A/B antibody., Immunohistochemistry analysis of paraffin-embedded rat brain using Ubiquitin Conjugating Enzyme E2 A/B antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded rat brain using Ubiquitin Conjugating Enzyme E2 A/B antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded mouse brain using Ubiquitin Conjugating Enzyme E2 A/B antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: UBE2A; RAD6A; Ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2B; RAD6B; Ubiquitin-conjugating enzyme E2 B; RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B

Immunogen: Synthesized peptide derived from the C-terminal region of human UBE2A/B.

Conjugate: Unconjugated

Modification: Unmodified