UCN (A103) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1488-50, BS1488-100

Sizes: 50µl, 100µl

Swiss-Prot: P55089

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Urocortins 1, 2 and 3 are the mammalian homologs of fish urotensin I. Mammalian urocortin is a member of the corticotropin-releasing hormone (CRH) family that is expressed in a region of the rat midbrain. The distinct expression pattern in discrete brain regions suggests that it influences such behavior as feeding, anxiety and auditory processing. Synthetic human urocortin binds with high affinity to CRH receptors and acts in vitro to release ACTH from dispersed rat anterior pituitary cells. It is suggested that urocortin may be an endogenous CRF-like factor in the brain responsible for the effects of stress on appetite. The gene which encodes urocortin maps to human chromosome 2. Specifically, urocortin III is a specific ligand for CRF type 2 receptor which mediates stress-coping responses. Human urocortin I and III are expressed in the human heart. Urocortin III is also expressed in the pituitary gland, adrenal gland, GI tract, ovary, spleen, brain and kidney.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Urocortin (A103) polyclonal antibody detects endogenous levels of Urocortin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of UCN (A103) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate, Immunohistochemistry (IHC) analyzes of UCN (A103) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 13 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Urocortin

Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human UCN.

Conjugate: Unconjugated

Modification: Unmodified