Cytokeratin 16 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90377-50, BS90377-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08779(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cytokeratin 16 polyclonal antibody detects endogenous levels of Cytokeratin 16 protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution., ICC staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CK-16, K16, CK 16, CK-16, CK16, Cytokeratin-16, Cytokeratin16, FNEPPK, Focal non epidermolytic palmoplantar keratoderma, K 16, K16, K1C16, K1CP, Keratin 1 type I, Keratin 16, Keratin, Keratin type I cytoskeletal 16, Keratin-16, Keratin16, KRT 16, Krt16, KRT16A, NEPPK, PC1, type I cytoskeletal 16,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified