EFP (D222) Polyclonal Antibody

Catalogue Numbers: BS2058-50, BS2058-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14258

Host: Rabbit

Reactivity: Human

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EFP (D222) polyclonal antibody detects endogenous levels of EFP protein.

Extra Notes: Western blot (WB) analysis of EFP (D222) pAb at 1:1000 dilution
Lane1:H1792 whole cell lysate(10ug)
Lane2:HepG2 whole cell lysate(10ug)
Lane3:MCF-7 whole cell lysate(10ug)
Lane4:L02 whole cell lysate(10ug)

Bioworld Molecular Weight: ~ 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; TRIM25; EFP; RNF147; ZNF147

Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human EFP.

Conjugate: Unconjugated

Modification: Unmodified