IL15 Polyclonal Antibody

Catalogue Numbers: BS60967-50, BS60967-100

Sizes: 50µl, 100µl

Alternative Name: Interleukin-15; IL-15; IL15

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40933

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Interleukin-15 (IL-15), also designated IL-T, is a cloned cytokine which shares several biological activities but no sequence homology with IL-2. Human, mouse and simian IL-15 cDNA clones have been isolated and characterized. All 3 species encode a 162 amino acid residue precursor protein containing a 48 amino acid leader that is cleaved to generate the mature form of IL-15. IL-15 stimulates the proliferation of T cells and NK cells, while enhancing B cell expansion and antibody production. Unlike IL-2, IL-15 is not produced by lymphocytes, but appears to be produced by macrophages, epithelial lines, muscle and placenta. IL-15 has also been shown to be a chemoattractant for human blood T lymphocytes and to be able to induce lymphokine-activated killer (LAK) activity in NK cells as well as to be able to induce the generation of cytolytic effector cells. Studies have shown that IL-15 is the only other cytokine that shares the β signaling subunit of the IL-2R. Evidence also suggests that like IL-2, IL-4 and IL-7, IL-15 utilizes the common IL-2Rγ subunit.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IL15 pAb detects endogenous levels of IL15 protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IL15 pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate(40ug)
Lane 2: HuT78 whole cell lysate(40ug)
Lane 3: Myla2059 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding Human IL15.

Conjugate: Unconjugated

Modification: Unmodified