Rat NPHN (Nephrin) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-R2406-24, E-EL-R2406-48, E-EL-R2406-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: NPHN

UNIProt ID: Q9R044

Target Synonyms: NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; renal glomerulus-specific cell adhesion receptor;

Research Areas: Cancer, Signal transduction

Target Species: Rat

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat NPHN concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NPHN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NPHN. You can calculate the concentration of Rat NPHN in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Rat NPHN in samples. No significant cross-reactivity or interference between Rat NPHN and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag