PCAF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91033-50, BS91033-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92831(Human) Q568Z2(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, , FC

All Applications: WB:1:500-1:2,000
FC:1:50-1:100
IP:1:10-1:50

Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessiblity to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PCAF polyclonal antibody detects endogenous levels of PCAF protein.

Bioworld Molecular Weight: 93 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PCAF on rat kidney tissue (1) and K562 cell (2) lysate using anti-PCAF antibody at 1/500 dilution., Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: Histone acetylase PCAF, P/CAF, KAT2B, PCAF, CAF, CREBBP associated factor, GCN5, GCN5L, GCN5L1, Histone acetylase PCAF, Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, KAT2B, KAT2B, Lysine acetyltransferase 2B, P, P/CAF, p300/CBP associated factor, P300/CBP-associated factor, Pcaf,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified