MRPL54 Polyclonal Antibody

Catalogue Numbers: BS65023-50, BS65023-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q6P161

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRPL54 polyclonal antibody detects endogenous levels of MRPL54 protein.

Extra Notes: Western blot (WB) analysis of MRPL54 polyclonal antibody at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:SGC7901 whole cell lysate(40ug)
Lane3:EC9706 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 39S ribosomal protein L54, mitochondrial; L54mt; MRP-L54; Mitochondrial large ribosomal subunit protein mL54; MRPL54

Immunogen: Synthetic peptide, corresponding to Human MRPL54.

Conjugate: Unconjugated

Modification: Unmodified