Pan-Succinyl-K Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79342-50, BS79342-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Succinylation of protein is a kind of post-translational modification of protein mainly occurring on lysine residues. Compared with methylation and acetylation, succinylation of lysine has a greater effect on protein structure and function. Sirt5 is an important de-succinylation modifying enzyme involved in the regulation of a variety of biological processes, such as reactive oxygen species defense, fatty acid metabolism and cell apoptosis

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Other Modified

Bioworld Molecular Weight: 10-150KDa

Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylatedY.

Conjugate: Unconjugated

Modification: Other Modified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Pan-Succinyl-K antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.