PIM1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9950M-50, BS9950M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11309

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: Pim-1 is a serine/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of PIM1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PIM1 Rabbit mAb at 1:1000 dilution
Lane 1: BT-20 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: SW480 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody.Counter stained with hematoxylin., ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Serine/threonine-protein kinase pim-1; Pim1; Pim-1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified