Human TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9058-48, ELK9058-96, ELK9058-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TPO Ab; TPO-Ab

Assay Type: Sandwich

Sensitivity: 1.14 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only