ATG2A Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71850-50, BS71850-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q2TAZ0

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Lipid transfer protein involved in autophagosome assembly. Tethers the edge of the isolation membrane (IM to the endoplasmic reticulum (ER and mediates direct lipid transfer from ER to IM for IM expansion. Binds to the ER exit site (ERES, which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B to the IM for membrane expansion. Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P-containing membranes. Also regulates lipid droplets morphology and distribution within the cell.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ATG2A Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Bioworld Molecular Weight: 213kDa

Specificity: Polyclonal Antibodies

Alternative Name: ATG2A

Immunogen: A synthetic peptide of human ATG2A(NP_055919.2).

Conjugate: Unconjugated

Modification: Unmodified