Actinin-α2/3 (Q58) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1984-50, BS1984-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35609/Q08043

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Actinin-α2/3 (Q58) polyclonal antibody detects endogenous levels of actinin-α2 and actinin-α3 protein.

Bioworld Molecular Weight: ~ 103 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Actinin-α2/3 (Q58) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of Actinin-α2/3 (Q58) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:5000. Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Actinin-α2/3 (Q58) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase, Immunohistochemistry (IHC) analyzes of Actinin-α2/3 (Q58) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; α-Actinin2; Actinin α2; Actininα2; αActinin-2; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; α-Actinin3; Actinin α3; Actininα3; αActinin-3; Actinin-α3; α-actinin-2; α-actinin-3

Immunogen: Synthetic peptide, corresponding to amino acids 30-70 of Human Actinin-α2.

Conjugate: Unconjugated

Modification: Unmodified