ZC3H8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS5770-50, BS5770-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8N5P1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (by similarity). ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ZC3H8 polyclonal antibody detects endogenous levels of ZC3H8 protein.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ZC3H8 pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (20ug)
Lane 2: Hela whole cell lysate (20ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human rectum carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Zinc finger CCCH domain-containing protein 8; ZC3HDC8

Immunogen: Synthetic peptide, corresponding to amino acids 133-178 of Human ZC3H8.

Conjugate: Unconjugated

Modification: Unmodified