β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K785-M-48, E-BC-K785-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: β-HB

Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: serum, plasma, urine, animal tissue

Assay Time: 60 min

Detection Type: Colorimetric method

Sensitivity: 0.01 mmol/L

Detection Range: 0.01-2.00 mmol/L

Test Principle: β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.

Inter CV: 4

Intra CV: 2

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag