Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Sizes: 48T, 96T, 500 Assays

Catalogue Numbers: E-BC-K235-M-48, E-BC-K235-M-96, E-BC-K235-M-500

Citations, Manuals and MSDS Available upon request.

Target Synonym: ALT;GPT

Research Areas: Liver and Renal Function

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: Serum, plasma, animal tissue, cells, cell culture supernatant

Assay Time: 80 min

Detection Type: Colorimetric method

Sensitivity: 0.75 IU/L

Detection Range: 0.75-72.3 IU/L

Test Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Inter CV: 9

Intra CV: 5.3

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag