POU5F1 / OCT4 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0046-50, MB0046-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20263

Host: Mouse

Reactivity: Mouse

Applications: WB

All Applications: WB: 1:1000

Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotenc

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of POU5F1/OCT4 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size:45KDa
Observed band size:45KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western BlotDetection of POU5F1 antibody in 20ug and 40ug mESC cell lysates using POU5F1 antibody (1:1000 diluted).

Alternative Name: POU domain, class 5, transcription factor 1; NF-A3; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; Pou5f1; Oct-3; Oct-4; Otf-3; Otf3

Immunogen: Recombinant full length Human POU5F1.

Conjugate: Unconjugated

Modification: Unmodified