HBC599 is a HBC analog. HBC is nonfluorescent in solution, but emits strong fluorescence upon forming tight complex with Pepper RNA aptamer. HBC-Pepper complex can be used to visualize RNA dynamics in live cells.

Product information

CAS Number: 2530162-00-4

Molecular Weight: 359.44

Formula: C21H17N3OS

Chemical Name: (Z)-4-(1-cyano-2-(6-((2-hydroxyethyl)(methyl)amino)benzo[b]thiophen-2-yl)vinyl)benzonitrile

Smiles: CN(CCO)C1=CC2SC(=CC=2C=C1)/C=C(\C#N)/C1C=CC(=CC=1)C#N

InChiKey: WXTHEUULEXARCU-WOJGMQOQSA-N

InChi: InChI=1S/C21H17N3OS/c1-24(8-9-25)19-7-6-17-10-20(26-21(17)12-19)11-18(14-23)16-4-2-15(13-22)3-5-16/h2-7,10-12,25H,8-9H2,1H3/b18-11+

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Pepper can activate the fluorescence of HBC. Mammalian cells expressing Pepper exhibits robust greenyellowish fluorescence on incubation with 1 μM HBC. Peppers allow simple and robust imaging of diverse RNA species in live cells with minimal perturbation of the target RNA’s transcription, localization and translation.

Products are for research use only. Not for human use.